Effect of different cardioprotective methods on extracorporeal circulation in fetal sheep: a randomized controlled trial

Abstract Background Congenital heart disease is a leading cause of death in newborns and infants. The feasibility fetal cardiac surgery linked to extracorporeal circulation (ECC); therefore, cardioplegic solutions need be effective long-lasting. Methods Eighteen pregnant sheep were divided into an ECC-only group, St. Thomas’ Hospital solution (STH1) group (STH group), HTK preservation (Custodiol®) (HTK group). Markers myocardial injury including troponin I (cTnI), T (cTnT) creatine kinase band (CKMB) measured at specific time points (T1: pre-ECC, T2: 30 min ECC, T3: 60 T4: post-ECC, T5: 120 post-ECC). Myocardial tissue was removed from the T5, apoptosis detected by TUNEL staining. Results Changes serum cTnI, cTnT CKMB concentrations not significantly different among three groups before during ECC(T1,T2,T3). At after ECC shutdown(T4), cTnI higher STH than start ECC. concentration groups. group. shutdown(T5), cTnT, number TUNEL-positive cells There no statistically significant difference rate mean arterial pressure Conclusion better STH1 reducing release cardiomyocyte markers apoptotic Fetal receiving had advantage all indicators, which suggests feasible.